Membrane:Article Title: Overexpression of the inwardly rectifying potassium channel Kir4.1 or Kir4.1 Tyr 9 Asp in Müller cells exerts neuroprotective effects in an experimental glaucoma model
Article Snippet: .. The primary antibodies used in this study included: mouse anti-GFAP (1:500, Sigma-Aldrich, St. Louis, MO, USA, Cat# G6171, RRID: AB_1840893), rabbit anti-Kir4.1 (1:400, Alomone, Jerusalem, Israel, Cat# APC-035, RRID: AB_2040120; APC-165 for membrane protein, RRID: AB_2041043), anti-B cell lymphoma/leukemia-2 (Bcl-2; 1:1000, Abclonal Biotechnology Co., Ltd, Wuhan, Hubei, China, Cat# A0208, RRID: AB_2757022), rabbit anti-Bcl-2-associated X protein (Bax; 1:1000, Abcam, Cambridge, MA, USA, Cat# ab32503, RRID: AB_725631), anti-myeloid differentiation primary response protein 88 (MYD88; 1:1000, Novus Biologicals, Littleton, CO, USA, Cat# NB100-56698SS, RRID: AB_838599), anti-nuclear factor kappa B p65 (NF-κB p65; 1:1000, Cell Signaling Technology, Danvers, MA, USA, Cat# 3033T, RRID: AB_331284), goat anti-translocator protein (TSPO; 1:1000, Novus Biologicals, Cat# NB100-41398, RRID: AB_788260), rabbit anti-cleaved caspase 3 (1:500, Zen-Bioscience, Chengdu, Sichuan, China, Cat# 341034, RRID: AB_2924432), rabbit anti-IL-1 receptor-associated kinase 1 (IRAK1; 1:1000, Abcam, Cat# ab180747, RRID: AB_2895218), rabbit anti-TNF receptor-associated factor 6 (TRAF6; 1:1000, Abcam, Cat# ab33915, RRID: AB_778572), mouse anti-β-actin (1:10,000, Sigma-Aldrich, Cat# A5441, RRID: AB_476744), rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH; 1:10,000, Sigma-Aldrich, Cat# G9545, RRID: AB_796208), and rabbit anti-Na-K ATPase (1:8000, Abcam, Cat# ab167390, RRID: AB_2890241). .. The secondary antibodies used were horseradish peroxidase (HRP)-conjugated donkey anti-mouse (1:10,000, Jackson ImmunoResearch Labs, West Grove, PA, USA, Cat# 715-035-150, RRID: AB_2340770), HRP-conjugated donkey anti-rabbit (1:10,000, Jackson ImmunoResearch Labs, Cat# 711-035-152, RRID: AB_10015282), and HRP-conjugated donkey anti-goat (1:10,000, Jackson ImmunoResearch Labs, Cat# 705-035-147, RRID: AB_2313587).
Article Title: Overexpression of the inwardly rectifying potassium channel Kir4.1 or Kir4.1 Tyr9 Asp in Müller cells exerts neuroprotective effects in an experimental glaucoma model
Article Snippet: The proteins were separated by SDS-PAGE using a Mini Protean 3 electrophoresis system (Bio-Rad, Hercules, CA, USA) and then transferred to a polyvinylidene fluoride (PVDF) membrane (R&D, Minneapolis, MN, USA) using a Mini Transblot electrophoretic transfer system (Bio-Rad). .. The primary antibodies used in this study included: mouse anti-GFAP (1:500, SigmaAldrich, St. Louis, MO, USA, Cat# G6171, RRID: AB_1840893), rabbit anti-Kir4.1 (1:400, Alomone, Jerusalem, Israel, Cat# APC035, RRID: AB_2040120; APC-165 for membrane protein, RRID: AB_2041043), anti-B cell lymphoma/leukemia-2 (Bcl-2; 1:1000, Abclonal Biotechnology Co., Ltd, Wuhan, Hubei, China, Cat# A0208, RRID: AB_2757022), rabbit anti-Bcl-2-associated X protein (Bax; 1:1000, Abcam, Cambridge, MA, USA, Cat# ab32503, RRID: AB_725631), anti-myeloid differentiation primary response protein 88 (MYD88; 1:1000, Novus Biologicals, Littleton, CO, USA, Cat# NB100-56698SS, RRID: AB_838599), anti-nuclear factor kappa B p65 (NF-κB p65; 1:1000, Cell Signaling Technology, Danvers, MA, USA, Cat# 3033T, RRID: AB_331284), goat antitranslocator protein (TSPO; 1:1000, Novus Biologicals, Cat# NB100-41398, RRID: AB_788260), rabbit anti-cleaved caspase 3 (1:500, Zen-Bioscience, Chengdu, Sichuan, China, Cat# 341034, RRID: AB_2924432), rabbit anti-IL-1 receptor-associated kinase 1 (IRAK1; 1:1000, Abcam, Cat# ab180747, RRID: AB_2895218), rabbit anti-TNF receptor-associated factor 6 (TRAF6; 1:1000, Abcam, Cat# ab33915, RRID: AB_778572), mouse anti-β-actin (1:10,000, Sigma-Aldrich, Cat# A5441, RRID: AB_476744), rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH; 1:10,000, Sigma-Aldrich, Cat# G9545, RRID: AB_796208), and rabbit anti-Na-K ATPase (1:8000, Abcam, Cat# ab167390, RRID: AB_2890241). .. The primary antibodies used in this study included: mouse anti-GFAP (1:500, SigmaAldrich, St. Louis, MO, USA, Cat# G6171, RRID: AB_1840893), rabbit anti-Kir4.1 (1:400, Alomone, Jerusalem, Israel, Cat# APC035, RRID: AB_2040120; APC-165 for membrane protein, RRID: AB_2041043), anti-B cell lymphoma/leukemia-2 (Bcl-2; 1:1000, Abclonal Biotechnology Co., Ltd, Wuhan, Hubei, China, Cat# A0208, RRID: AB_2757022), rabbit anti-Bcl-2-associated X protein (Bax; 1:1000, Abcam, Cambridge, MA, USA, Cat# ab32503, RRID: AB_725631), anti-myeloid differentiation primary response protein 88 (MYD88; 1:1000, Novus Biologicals, Littleton, CO, USA, Cat# NB100-56698SS, RRID: AB_838599), anti-nuclear factor kappa B p65 (NF-κB p65; 1:1000, Cell Signaling Technology, Danvers, MA, USA, Cat# 3033T, RRID: AB_331284), goat antitranslocator protein (TSPO; 1:1000, Novus Biologicals, Cat# NB100-41398, RRID: AB_788260), rabbit anti-cleaved caspase 3 (1:500, Zen-Bioscience, Chengdu, Sichuan, China, Cat# 341034, RRID: AB_2924432), rabbit anti-IL-1 receptor-associated kinase 1 (IRAK1; 1:1000, Abcam, Cat# ab180747, RRID: AB_2895218), rabbit anti-TNF receptor-associated factor 6 (TRAF6; 1:1000, Abcam, Cat# ab33915, RRID: AB_778572), mouse anti-β-actin (1:10,000, Sigma-Aldrich, Cat# A5441, RRID: AB_476744), rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH; 1:10,000, Sigma-Aldrich, Cat# G9545, RRID: AB_796208), and rabbit anti-Na-K ATPase (1:8000, Abcam, Cat# ab167390, RRID: AB_2890241).
Cell-Signaling:Article Title: Overexpression of the inwardly rectifying potassium channel Kir4.1 or Kir4.1 Tyr 9 Asp in Müller cells exerts neuroprotective effects in an experimental glaucoma model
Article Snippet: .. The primary antibodies used in this study included: mouse anti-GFAP (1:500, Sigma-Aldrich, St. Louis, MO, USA, Cat# G6171, RRID: AB_1840893), rabbit anti-Kir4.1 (1:400, Alomone, Jerusalem, Israel, Cat# APC-035, RRID: AB_2040120; APC-165 for membrane protein, RRID: AB_2041043), anti-B cell lymphoma/leukemia-2 (Bcl-2; 1:1000, Abclonal Biotechnology Co., Ltd, Wuhan, Hubei, China, Cat# A0208, RRID: AB_2757022), rabbit anti-Bcl-2-associated X protein (Bax; 1:1000, Abcam, Cambridge, MA, USA, Cat# ab32503, RRID: AB_725631), anti-myeloid differentiation primary response protein 88 (MYD88; 1:1000, Novus Biologicals, Littleton, CO, USA, Cat# NB100-56698SS, RRID: AB_838599), anti-nuclear factor kappa B p65 (NF-κB p65; 1:1000, Cell Signaling Technology, Danvers, MA, USA, Cat# 3033T, RRID: AB_331284), goat anti-translocator protein (TSPO; 1:1000, Novus Biologicals, Cat# NB100-41398, RRID: AB_788260), rabbit anti-cleaved caspase 3 (1:500, Zen-Bioscience, Chengdu, Sichuan, China, Cat# 341034, RRID: AB_2924432), rabbit anti-IL-1 receptor-associated kinase 1 (IRAK1; 1:1000, Abcam, Cat# ab180747, RRID: AB_2895218), rabbit anti-TNF receptor-associated factor 6 (TRAF6; 1:1000, Abcam, Cat# ab33915, RRID: AB_778572), mouse anti-β-actin (1:10,000, Sigma-Aldrich, Cat# A5441, RRID: AB_476744), rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH; 1:10,000, Sigma-Aldrich, Cat# G9545, RRID: AB_796208), and rabbit anti-Na-K ATPase (1:8000, Abcam, Cat# ab167390, RRID: AB_2890241). .. The secondary antibodies used were horseradish peroxidase (HRP)-conjugated donkey anti-mouse (1:10,000, Jackson ImmunoResearch Labs, West Grove, PA, USA, Cat# 715-035-150, RRID: AB_2340770), HRP-conjugated donkey anti-rabbit (1:10,000, Jackson ImmunoResearch Labs, Cat# 711-035-152, RRID: AB_10015282), and HRP-conjugated donkey anti-goat (1:10,000, Jackson ImmunoResearch Labs, Cat# 705-035-147, RRID: AB_2313587).
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